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Immunohistochemistry example image

Fluorescent immunostaining of 0-21 day old mouse trachea epithelium. Cy3 conjugated acetylated tubulin antibody delineates cilia (red) while Alexa-Fluor 488 conjugated phalloidin delineated epithelial cell outlines (green). Single colour images are collapsed volume deconvoluted z-stacks.
All scale bars =10 um.
Image taken from Francis et al. The initiation and maturation of cilia generated flow in the newborn and postnatal mouse airway: American Journal of Physiology - Lung Cellular & Molecular Physiology: (April 3, 2009). In Press (PDF)