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EFIC example

Episcopic fluorescence image capture (EFIC) z-stack of a normal E9.5 mouse embryo. Autofluorescent images were collected from the top of a wax embedded specimen undergoing microtome sectioning and compiled into a single z-stack. (for review see: Rapid high resolution three dimensional reconstruction of embryos with episcopic fluorescence image capture. Birth Defects Research Part C, Embryo Today: Reviews 72: 213-223, 2004.).