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TIRF example (Quicktime movie)

A Two color TIRF time lapse movie showing a Cx43FL-DsRed plaque being transported to the cell membrane along a microtubule (from bottom to top of movie) in a NIH3T3 cell. Green fluorescence is GFP-tubulin, red fluorescence is Cx43-DsRed. Frames were collected at 2.7 fps, playback is at 10 fps.
Scale bar = 5 um.
Movie taken from Francis RJ et al. Connexin43 modulates cell polarity and directional cell migration by regulating microtubule Dynamics. unpublished manuscript.