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Epifluorescent time-lapse example (Quicktime movie)


Epifluorescent time-lapse movie of primordial germ cell migration in the whole mount tail section of a ~E8.0 mouse embryo. Primordial germ cells were labeled with a Oct4-GFP label. Individual frames were collected 6 minutes apart and are composites of collapsed volume deconvoluted z-stacks imaged on a Leica DMLFSA microscope with 10X water immersion lens.
Movie taken from Francis RJ and Lo CW. Primordial germ cell deficiency in the connexin 43 knockout mouse arises from apoptosis associated with abnormal p53 activation. Development 2006;133(17):3451-60.