Richards Imaging Experience
Richards biomedical imaging related experience can be broadly divided into the following areas (url links are provided for examples in each area):
1)
Standard Light microscopy (DIC/Phase/fluorescent) and image processing
(Volume deconvolution).
a.
Phase-contrast and DIC example
images
b.
Immunohistochemistry example image
2) Laser based imaging: Confocal microscopy: Total internal reflection fluorescence (TIRF) microscopy. High-speed two colour TIRF microscopy
a.
TIRF example
b.
TIRF example (Quicktime movie)
3) Three dimensional imaging and volume reconstruction: Computed tomography (CT) imaging, and episcopic fluorescence imaging (EFIC) (for review see: Rapid high resolution three dimensional reconstruction of embryos with episcopic fluorescence image capture. Birth Defects Research Part C, Embryo Today: Reviews 72: 213-223, 2004.),
4) Live cell/tissue imaging: time-lapse imaging of cell motility in tissue Explants and whole mount tissue sections (Light/Fluorescence). High-speed imaging of cilia beat dynamics and measurement of cilia generated flow (fluorescent beads).
a.
Cell migration time-lapse example (Quicktime movie)
b.
Epifluorescent time-lapse example (Quicktime movie)
c.
High-speed imaging example (Quicktime movie)
d.
Imaging cilia generated flow
(Quicktime movie)
e.
High-speed imaging example 2
(Quicktime movie)
f.
Cilia activity in the
embryonic node (Quicktime movie)
g.
Cilia generated flow within
the embryonic node (Quicktime movie)
h.
Live embryo imaging 1
(Quicktime movie)
i.
Live embryo imaging 2
(Quicktime movie)
5) Specialty imaging techniques. Electrophysiology dye injection to quantify gap-junctional communication between cells.